Skip to main content
Fig. 6 | Journal of Biological Engineering

Fig. 6

From: Respiration activity monitoring system for any individual well of a 48-well microtiter plate

Fig. 6

Comparison of E. coli BL21 (DE3) pRotHi-YFP cultivations using the newly introduced μRAMOS MTP system (─■─) and the standard RAMOS shake flask system (──). Mean values of the measured oxygen transfer rates of 48 wells and 4 shake flask cultivations are shown (a). b Detailed propagation of corresponding standard deviations of the measured oxygen transfer rates of the 48 wells and 4 shake flask and cultivations. Cultivation medium: Synthetic Wilms-MOPS auto-induction medium with 0.55 g L-1 glucose, 2 g L-1 lactose and 5 g L-1 glycerol. μRAMOS (MTP) cultivation conditions: 48-well Round Well Plate without optodes, V L  = 800 μL, n = 1000 rpm, shaking diameter d 0  = 3 mm, 37 °C, flow phase + high flow phase: 17 min, stop phase: 3 min., standard RAMOS cultivation conditions: 250 mL RAMOS shake flask, V L  = 10 mL, n = 350 rpm, d 0  = 50 mm, 37 °C, flow phase + high flow phase: 25 min, stop phase: 5 min

Back to article page