Fig. 7
From: Respiration activity monitoring system for any individual well of a 48-well microtiter plate
Respiration activity of parsley cell suspension cultures in μRAMOS (MTP) and RAMOS (shake flask) treated with salicylic acid (SA) and a 13 amino-acid defense elicitor of Phytophthora sojae (Pep13). Arrows indicate the addition of 100 μM salicylic acid (SA, 72 h) and of a 13 amino-acid defense elicitor of Phytophthora sojae (Pep13, 96 h). Shadows in (a) symbolize standard deviations of eight cultivations. (b) Single shake flask cultivations. Cultivation medium: Gamborg B5 medium with 20 g L-1 sucrose. Cultivation conditions μRAMOS (a) 48-well Round Well Plate without optodes, V L = 2000 μL, n = 800 rpm, shaking diameter d 0 = 3 mm, 25 °C, flow phase + high flow phase: 20 min, stop phase: 10 min, standard RAMOS cultivation conditions (b) 250 mL shake flask, V L = 50 mL, n = 180 rpm, d 0 = 50 mm, 25 °C, flow phase + high flow phase: 20 min, stop phase: 10 min