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Fig. 8 | Journal of Biological Engineering

Fig. 8

From: Respiration activity monitoring system for any individual well of a 48-well microtiter plate

Fig. 8

μRAMOS (a) and RAMOS (b) cultivation of H. polymorpha RB11 pC10-FMD (PFMD-GFP) under magnesium limitation. b republished data of Kottmeier et al. (2010) [33]. The percentages of magnesium are normalized to the original medium containing 3.0 g L-1 MgSO4 · 7H2O as 100 %. Mean values and corresponding standard deviations (colored shadows) of triple cultivations are shown in (a). Single cultivations are shown in (b). For clarity, only every second data point over time is indicated by the corresponding symbol in all curves. The cultivations containing 0.8 % magnesium were shifted for -1 h due to possible inoculation variances. Cultivation medium: Synthetic Syn-6-MES medium with 10 g L-1 glycerol. μRAMOS cultivation conditions (a): 48-well Round Well Plate without optodes, V L  = 800 μL, n = 1000 rpm, d 0  = 3 mm, 30 °C, flow phase + high flow phase: 16 min, stop phase: 4 min., standard RAMOS cultivation conditions (b): 250 mL RAMOS shake flask, V L  = 10 mL, n = 300 rpm, d 0  = 50 mm, 30 °C, flow phase + high flow phase: 20 min, stop phase: 10 min

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