Fig. 9
From: Respiration activity monitoring system for any individual well of a 48-well microtiter plate
48 different μRAMOS (a) and 8 different RAMOS (b) cultivations of H. polymorpha RB11 pC10-FMD (PFMD-GFP) under potassium limitation. b republished data of Kottmeier et al. (2010) [39]. The percentages of potassium are normalized to the original medium containing 1.0 g L-1 KH2PO4 and 3.3 g L-1 KCl as 100 %. Single cultivations are shown. For clarity, only the concentrations of the RAMOS cultivations are indicated by a corresponding symbol within the μRAMOS results. Cultivation medium: Synthetic Syn-6-MES medium with 10 g L-1 glycerol. μRAMOS cultivation conditions (a): 48-well Round Well Plate without optodes, V L = 800 μL, n = 1000 rpm, d 0 = 3 mm, 30 °C, flow phase + high flow phase: 16 min, stop phase: 4 min, standard RAMOS cultivation conditions (b): 250 mL RAMOS shake flask, V L = 10 mL, n = 300 rpm, d 0 = 50 mm, 30 °C, flow phase + high flow phase: 20 min, stop phase: 10 min