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Fig. 3 | Journal of Biological Engineering

Fig. 3

From: Can terminators be used as insulators into yeast synthetic gene circuits?

Fig. 3

Characterization of synthetic promoters made of a terminator placed in front of pCYC1noTATA. Fluorescence levels are normalized with respect to the fluorescent signal produced by the bare pCYC1noTATA. The negative control (background relative fluorescence) is also shown. The strong TATA boxes into DEG1t and Tsynth8 cause, respectively, a 6.6 and 5.1 fold increase in pCYC1noTATA fluorescence level. The weaker TATA box belonging to the genCYC1t has a lower effect (2.2 folds increase). The * symbol indicates a statistically significant difference with respect to the fluorescence level of the negative control

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