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Fig. 5 | Journal of Biological Engineering

Fig. 5

From: Can terminators be used as insulators into yeast synthetic gene circuits?

Fig. 5

Possible scenarios for promoter competition induced by the presence of a terminator-insulator. a A strong insulator efficiency element (EE) is placed in proximity of the promoter upstream activating sequence. DNA steric occupancy by RNA polymerase II at the EE prevents activator binding at the UAS and recruitment of other RNA polymerase II molecules to the promoter TATA box. b DNA bending–here due to the presence of an activator at its UAS–puts the EE spatially close to the TATA box causing a competition among RNA polymerase II molecules to get access to the EE and the TATA box themselves. DNA bending might also put the EE near the UAS provoking a competition (or even a collision) between RNA polymerase II and activator molecules

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