Fig. 8

Mutational study on DEG1t-pGPD and DEG1t-mut_pGPD. Similarly to pCYC1noTATA, the fluorescence level of the wild-type pGPD is restored by removing, via double mutations, the TATA-box-like motif from the DEG1t efficiency element. Moreover, DEG1t is proved to be able to reduce the already rather low fluorescence expressed by mut_pGPD, where the sequence of the strong bipartite UAS has been deeply modified to prevent GRF1 binding. Therefore, RNA polymerase II molecules recruited by DEG1t are able to interfere also with far downstream transcription activation processes. By substituting DEG1t with mut_DEG1t, fluorescence grows back to the original level of mut_pGPD. All fluorescence levels are normalized with respect to the wild-type pGPD one. The ∙ symbol indicates no statistically significant difference with respect to pGPD fluorescence level, whereas the ∘ symbol indicates no statistically significant difference with respect to mut_pGPD fluorescence level