Can terminators be used as insulators into yeast synthetic gene circuits?

Table 2 Structures of the five yeast promoters we insulated with DEG1t

Promoter	Length	UAS	Distance DEG1t(TATAAA)-UAS (nt)	Activator	TATA box
pGPD	680	–513	205	GRF1	–141
pTEF2	645	–456	226	GRF1	–127
pADH1	700	–694	43	GCR1	–128
pTEF1	401	–324	114	GRF1	–120
pACT1	489	–410	116	GRF2	–196

UAS and TATA box position refers to the ATG start codon. Only the leftmost UASes in the promoter sequences are here considered

ISSN: 1754-1611