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Fig. 2 | Journal of Biological Engineering

Fig. 2

From: Cellulolytic RoboLector – towards an automated high-throughput screening platform for recombinant cellulase expression

Fig. 2

Schematic comparison of conventional protocols and modified protocols adapted for HT application in MTPs. a Extraction of recombinant enzymes/proteins from E. coli by chemical cell lysis applying BugBuster® reagent. b Colorimetric AzoCMC assay for cellulase activity measurement. c PAHBAH test for quantification of reducing sugars after CMC hydrolysis

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