Fig. 4

Calibration curves for the Azo-CMC cellulase activity assay comparing the standard protocol and HT protocol at varied incubation times (according to Fig. 2b). Error bars indicate standard deviation of three simultaneously performed assays. Assay conditions: incubation for 10 min in 5 mL tubes, V_L = 1 mL, n = 900 rpm, d₀ = 3 mm (standard protocol). Incubation for 10/20 min in 96well DWP, V_L = 400 μL, n = 900 rpm, d₀ = 3 mm (HT protocol). 0.2 g L⁻¹ Azo-CMC in 0.1 M acetate buffer (pH = 4.8), endoglucanase (EGII) from Trichoderma longibrachiatum, 45 °C, absorbance measurement at 590 nm