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Fig. 7 | Journal of Biological Engineering

Fig. 7

From: Single cell isolation process with laser induced forward transfer

Fig. 7

The impact of glycerol concentration. The representative size distribution of the transferred cell at laser pulse energy of 7 μJ, spot size of 40 μm, thickness of Titanium of 60 nm working distance of 500 μm and no modification in receptor but adding to culture medium with different concentration of glycerol: a control (0%), b 4%, c 8% and d 12%, e the bar diagram of glycerol concentration as function of the number of transferred cells within 25 laser shots and f the proliferation ratio change of the of isolated cells with various glycerol concentration within 5 days incubating with no modified culture medium

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