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Table 1 The experimental arrangement of single factor experiments

From: Single cell isolation process with laser induced forward transfer

Parameters Values
Laser pulse energy (E) 1 μJ, 3 μJ, 5 μJ, 7 μJ, 9 μJ, 11 μJ, 13 μJ, 15 μJ
Laser spot sizea(Diameter, D) 20 μm, 25 μm, 30 μm, 35 μm, 40 μm, 45 μm,50 μm, 55 μm, 60 μm, 65 μm
Thickness of Titanium (T -t ) 20 nm, 40 nm, 60 nm, 80 nm, 100 nm, 120 nm, 140 nm
Working distance (l) 0 μm, 100 μm, 200 μm, 300 μm, 400 μm, 500 μm, 600 μm, 700 μm
Concentration of Glycerol (d) 0%, 2%, 4%, 6%, 8%, 10%, 12%, 14%
Thickness of Alginate (T -A ) 0 μm, 2 μm, 4 μm, 6 μm, 8 μm, 10 μm
  1. aThe spot was in reality the functional zone on the surface of titanium, rather than the theoretical spot of the laser. It was altered by varying the focus away from the titanium surface