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Table 2 The result of orthogonal experiment

From: Single cell isolation process with laser induced forward transfer

No.

E /μJ

D /μm

T -t /nm

l/μm

No. of Cells

Peak Diametera

Proliferation Ratiob

1

5

45

80

500

24

25

24

16

16

17

1.47

1.48

1.46

2

5

30

40

300

21

22

22

15

14

14

1.3

1.32

1.31

3

9

60

40

500

18

16

19

13

13

13

1.33

1.33

1.32

4

9

30

80

700

35

37

37

15

13

15

1.35

1.32

1.36

5

5

60

120

700

16

14

14

19

18

19

1.52

1.54

1.49

6

9

45

120

300

24

25

24

15

16

16

1.41

1.46

1.44

7

13

45

40

700

31

29

33

11

12

12

1.12

1.09

1.07

8

13

60

80

300

47

46

38

13

13

14

1.26

1.18

1.22

9

13

30

120

500

42

38

40

12

12

11

1.13

1.11

1.07

Mean I

20.223

32.667

23.447

19.777

No. of Cells

Mean II

26.110

26.553

24.663

27.333

Mean III

28.110

15.223

26.333

27.333

Range

7.887

17.444

2.886

7.556

Mean I

16.443

13.443

13.000

14.443

Peak Diameter

Mean II

14.333

14.557

14.663

13.667

Mean III

12.223

15.000

15.337

14.890

Range

4.200

1.557

2.337

1.223

Mean I

1.433

1.290

1.243

1.323

Proliferation Ratio

Mean II

1.370

1.333

1.343

1.340

Mean III

1.177

1.357

1.393

1.317

Range

0.256

0.067

0.150

0.023

  1. aThe size distribution of transferred cell was fitting with Gaussian curve, and the peak size was defined as peak diameter with unit of μm
  2. bThe proliferation ratio here was the proliferation ration after 2 days post-transfer culturing with the consideration of cell’s self-recoverability