Skip to main content

Table 2 The result of orthogonal experiment

From: Single cell isolation process with laser induced forward transfer

No. E /μJ D /μm T -t /nm l/μm No. of Cells Peak Diametera Proliferation Ratiob
1 5 45 80 500 24 25 24 16 16 17 1.47 1.48 1.46
2 5 30 40 300 21 22 22 15 14 14 1.3 1.32 1.31
3 9 60 40 500 18 16 19 13 13 13 1.33 1.33 1.32
4 9 30 80 700 35 37 37 15 13 15 1.35 1.32 1.36
5 5 60 120 700 16 14 14 19 18 19 1.52 1.54 1.49
6 9 45 120 300 24 25 24 15 16 16 1.41 1.46 1.44
7 13 45 40 700 31 29 33 11 12 12 1.12 1.09 1.07
8 13 60 80 300 47 46 38 13 13 14 1.26 1.18 1.22
9 13 30 120 500 42 38 40 12 12 11 1.13 1.11 1.07
Mean I 20.223 32.667 23.447 19.777 No. of Cells
Mean II 26.110 26.553 24.663 27.333
Mean III 28.110 15.223 26.333 27.333
Range 7.887 17.444 2.886 7.556
Mean I 16.443 13.443 13.000 14.443 Peak Diameter
Mean II 14.333 14.557 14.663 13.667
Mean III 12.223 15.000 15.337 14.890
Range 4.200 1.557 2.337 1.223
Mean I 1.433 1.290 1.243 1.323 Proliferation Ratio
Mean II 1.370 1.333 1.343 1.340
Mean III 1.177 1.357 1.393 1.317
Range 0.256 0.067 0.150 0.023
  1. aThe size distribution of transferred cell was fitting with Gaussian curve, and the peak size was defined as peak diameter with unit of μm
  2. bThe proliferation ratio here was the proliferation ration after 2 days post-transfer culturing with the consideration of cell’s self-recoverability