Fig. 3
Effect of 18 nm surface-coated gold NPs on Aβ1–40 monomer aggregation. Aβ1–40 monomer diluted to 40 μM in 40 mM Tris–HCl (pH 8.0) was aggregated alone (control, Ο) or in the presence of surface-coated NPs. a Aggregation in the presence of 200 pM NPs displaying citrate (■), PAA (▲), or PAH (♦) surface chemistries. b Aggregation in the presence of 200 pM (♦), 100 pM (
), or 20 pM (
) PAH-coated NPs. Monomer aggregation was induced by continuous agitation and monitored periodically via ThT fluorescence. ThT fluorescence, expressed as the fraction of the control equilibrium plateau, is plotted versus relative time, which is the fraction of the control lag time. Results are representative of 3–5 independent experiments