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Fig. 2 | Journal of Biological Engineering

Fig. 2

From: Synthetic photosynthetic consortia define interactions leading to robustness and photoproduction

Fig. 2

S. elongatus supports microbial communities in batch culture. Batch cultures of cscB + S. elongatus (green) in co-culture with B. subtilis (blue), S. cerevisiae (purple), or E. coli (orange) were grown in constant light. CscB + S. elongatus cells/mL were determined by flow cytometry every 12 hours for co-cultures containing B. subtilis (a; green), S. cerevisiae (b, d; green), and E. coli (e, f; green). Co-cultures with uninduced (dashed lines) or induced cscB expression (solid lines) were tested. Heterotroph viability was monitored by colony forming unit (CFU) for all B. subtilis (a; blue), S. cerevisiae (b strain W303, d strain W303Clump; purple), and E. coli (e; strain W, f; strain W ΔcscR; orange) co-cultures. Data for abde, and f, are representative, same-day experiments where error bars are the standard error in 3 biological replicates. Additional replicates in Additional file 1: Figure S2. c Axenic heterotroph growth was tested in defined media with varying concentrations; the range of sucrose that cscB + S. elongatus can secrete in 48 hours is denoted by a green box. Average OD600 is shown as a metric of growth for ≥ 6 biological replicates. OD600 was correlated to viable colony forming units (CFU) in Additional file 1: Figure S3. No contaminants/heterotrophic colonies grew from axenic cyanobacteria controls

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