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Fig. 2 | Journal of Biological Engineering

Fig. 2

From: Reliable measurement of E. coli single cell fluorescence distribution using a standard microscope set-up

Fig. 2

Schematic representation of the synthetic gene circuit used in this work. The expression of the green fluorescent protein (GFP) can be modulated transcriptionally, through the addition of IPTG, due to the presence of the Lac Operator O1. The circuit was cloned in a pSC101 low copy number plasmid and transformed in E. coli cells of the strain TOP 10 F’ overexpressing the Lac repressor

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Journal of Biological Engineering

ISSN: 1754-1611