Fig. 2From: Synthesis of umbelliferone derivatives in Escherichia coli and their biological activities a In vitro reaction mixture and b whole cells bioconversion of umbelliferone on the HPLC-PDA analysis. (i) control reaction of umbelliferone using E. coli BL21 (DE3); (ii) hydroxylation with M13 and (iii) CYP450 BM3 at 48Â h; (iv) glycosylation with YjiC at 12Â h; and (v) methylation with SaOMT2 at 48Â h, respectivelyBack to article page