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Table 3 Coded values and real values of the experimental variables for Box-Behnken method

From: Significantly enhanced biomass production of a novel bio-therapeutic strain Lactobacillus plantarum (AS-14) by developing low cost media cultivation strategy

  Independent Variables (g/l) Symbol Coded values
−1 (Low) 0 (Centre) 1 (High)
Production Medium Optimization Glucose (X1) 10 15 20
Yeast Extract (X 2) 4 6 8
Cheese Whey (X3) 45 60 75
(NH4)2SO4 (X4) 10 15 20
Corn steep Liquor (X5) 10 15 20
Operating Conditions Optimization pH (A) 6.2 6.3 6.4
Temperature °C (B) 35 40 45
  1. Other composition included sodium acetate (5g/l), MgSO4.7H2O (0.3g/l), MnSO4.4H2O (0.04g/l), Tween 80 0.2