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Table 4 Optimization of five nutrient variables (X1-X5) and two operating conditions (A, B) using Taugchi’s experimental design for the production of dry cell mass

From: Significantly enhanced biomass production of a novel bio-therapeutic strain Lactobacillus plantarum (AS-14) by developing low cost media cultivation strategy

Runs order Nutrient variables (g/l) and operating conditions levels a Response Dry cell mass (g/l)
X1 X 2 X3 X4 X5 A B
1 1 2 2 2 2 1 1 8.99 ± 0.02
2 3 1 3 4 2 2 1 9.12 ± 0.06
3 2 3 4 1 2 3 1 10.22 ± 0.04
4 4 1 4 2 3 4 1 13.47 ± 0.12
5 4 4 1 3 2 1 2 13.01 ± 0.01
6 3 2 2 3 1 2 2 8.21 ± 0.07
7 4 4 2 1 4 3 2 13.55 ± 0.01
8 2 1 3 3 4 4 2 11.32 ± 0.05
9 1 3 3 3 3 2 3 10.91 ± 0.05
10 2 2 4 4 3 2 3 11.21 ± 0.07
11 2 4 2 2 1 2 3 12.87 ± 0.06
12 1 4 2 4 4 2 3 12.71 ± 0.06
13 1 4 1 1 3 3 1 11.82 ± 0.02
14 4 3 1 4 3 3 3 13.88 ± 0.03
15 3 3 3 2 4 4 3 11.49 ± 0.08
16 1 1 2 1 1 3 4 8.21 ± 0.05
  1. The bold data represent that experiemental run which shows maximum L. Plantarum biomass production
  2. aX1, Glucose; X 2, Yeast extract; X3, Cheese Whey; X4, (NH4)2SO4; X5 corn steep liquor. Other composition included sodium acetate (5 g/l), MgSO4.7H2O (0.3 g/l), MnSO4.4H2O (0.04 g/l), Tween 80 (0.2 g/l)