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Table 4 Optimization of five nutrient variables (X1-X5) and two operating conditions (A, B) using Taugchi’s experimental design for the production of dry cell mass

From: Significantly enhanced biomass production of a novel bio-therapeutic strain Lactobacillus plantarum (AS-14) by developing low cost media cultivation strategy

Runs order

Nutrient variables (g/l) and operating conditions levels a

Response Dry cell mass (g/l)

X1

X 2

X3

X4

X5

A

B

1

1

2

2

2

2

1

1

8.99 ± 0.02

2

3

1

3

4

2

2

1

9.12 ± 0.06

3

2

3

4

1

2

3

1

10.22 ± 0.04

4

4

1

4

2

3

4

1

13.47 ± 0.12

5

4

4

1

3

2

1

2

13.01 ± 0.01

6

3

2

2

3

1

2

2

8.21 ± 0.07

7

4

4

2

1

4

3

2

13.55 ± 0.01

8

2

1

3

3

4

4

2

11.32 ± 0.05

9

1

3

3

3

3

2

3

10.91 ± 0.05

10

2

2

4

4

3

2

3

11.21 ± 0.07

11

2

4

2

2

1

2

3

12.87 ± 0.06

12

1

4

2

4

4

2

3

12.71 ± 0.06

13

1

4

1

1

3

3

1

11.82 ± 0.02

14

4

3

1

4

3

3

3

13.88 ± 0.03

15

3

3

3

2

4

4

3

11.49 ± 0.08

16

1

1

2

1

1

3

4

8.21 ± 0.05

  1. The bold data represent that experiemental run which shows maximum L. Plantarum biomass production
  2. aX1, Glucose; X 2, Yeast extract; X3, Cheese Whey; X4, (NH4)2SO4; X5 corn steep liquor. Other composition included sodium acetate (5 g/l), MgSO4.7H2O (0.3 g/l), MnSO4.4H2O (0.04 g/l), Tween 80 (0.2 g/l)