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Fig. 2 | Journal of Biological Engineering

Fig. 2

From: AAV-based dual-reporter circuit for monitoring cell signaling in living human cells

Fig. 2

Construction of AAV-based dual reporters and their functional characterization. a Schematic illustration of 3 AAV-based dual reporters used for system validation. All reporters have the basic configuration, including the AAV-related ITR sequences for AAV-production and packaging, the two reporter genes GFP and Luc separated by 2A sequences (self-cleavage peptide). The dual-reporters are either promoterless or under the control of mCMV or fCMV, as shown from top to bottom. bThe GFP expression (green) in HEK293 cells were recorded with a fluorescence microscope following transfections of either a promoterless, a mCMV, or a fCMV-driven reporter at 24 h (top panel), 48 h (middle panel) and 72 (lower panel). Arrows indicate GFP-positive cells. c The Luc expression in HEK293 cells were determined by luciferase assay following transfections of three reporters at same time points. The luciferase activities are expressed as relative light units (RLU), normalized against protein input, and presented as fold increase over untreated control (mean ± SD, n = 3)

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