Fig. 4

The specificity of AAV-based reporter using transduction protocol. a HEK293 cells were transduced with either NFκB (a) or AP-1 (b) reporter AAV (MOI = 1) for 24 h. Cells were then switched to low serum medium in the presence or absence of TNFα (10 ng/mL) or PMA (50 ng/mL) for 24 h. The expression of GFP (images, upper two panels) and Luc activities (the lower graph) were examined by fluorescence microscope or luciferase assay respectively. The luciferase activities are expressed as relative light units (RLU), normalized against protein input, and presented as fold increase over untreated control (mean ± SD, n = 3). *** P < 0.001, Student t-Test versus controls