Fig. 5
From: AAV-based dual-reporter circuit for monitoring cell signaling in living human cells
Dose-response of NF-κB reporter in HEK293 and MCF-7 cells. a Following transduction with NF-κB reporter virus (MOI = 1) for 24 h, HEK293 cells (a) or MCF-7 cells (c) were treated with increasing concentration of TNFα (0, 0.1, 1.0, 5.0, 10, and 50 ng/mL) for 24 h, and GFP expression was recorded. b The luciferase activities were determined by luciferase assay using cell lysates, and Luc activities expressed as relative light units (RLU), normalized against protein input, and presented as fold increase over untreated control (mean ± SD, n = 3)