Fig. 6
From: AAV-based dual-reporter circuit for monitoring cell signaling in living human cells
A time-course study of NF-kB reporter in transduced HEK293 cells. Following transduction with NF-κB reporter virus (MOI = 1) for 24 h, HEK293 cells were switched to low serum medium in the presence or absence of TNFα (10 ng/mL) for 0, 3, 7, 24, 48, and 72 h. a GFP expression for the TNFα treatment group. b. GFP expression for the untreated control group. c At the same time points, luciferase assays were conducted and luciferase activities were plotted as relative light units (RLU), normalized against protein input (mean ± SD, n = 3). *** P < 0.001, Student t-Test versus controls