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Fig. 1 | Journal of Biological Engineering

Fig. 1

From: Streamlining recombination-mediated genetic engineering by validating three neutral integration sites in Synechococcus sp. PCC 7002

Fig. 1

Schematic representation of the construction of neutral integration site strains. The genes in green arrows represent the genetic sites of interest. The BioBrick Prefix and Suffix are indicated in blue and the kanamycin resistance cassette in red. a, c, e Schematic representation of the genetic organization of wildtype chromosomal DNA Synechococcus sp. PCC 7002 at three NISs (not to scale). b Chromosomal DNA after homologous recombination replacing SYNPCC7002_A2842 with a kanamycin resistance cassette flanked by the BioBrick Prefix and Suffix. d Chromosomal DNA after homologous recombination inserting a kanamycin resistance cassette flanked by the BioBrick Prefix and Suffix between SYNPCC7002_A0935 and SYNPCC7002_A0936. f Chromosomal DNA after homologous recombination resulting in a disruption of SYNPCC7002_A0159 by inserting a kanamycin resistance cassette flanked by the BioBrick Prefix and Suffix

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Journal of Biological Engineering

ISSN: 1754-1611