Fig. 3

Results of the transformation efficiency assessment by camera-based plate imaging. Synechococcus sp. PCC 7002 strains were transformed with a kanamycin resistance cassette containing either 100, 200, 400 or 800 nt homology with the targeted genetic site in the chromosome to induce homologous recombination. Three different approaches were used to target three sites: (1) Insertion in the non-coding region between SYNPCC7002_A0935 and SYNPCC7002_A0936 (noted as A0935), (2) gene disruption of the coding region of desB (noted as A0159) and (3) gene replacement of glpK (noted as A2842). The intensities of spotted transformant colonies on plates of the constructed mutants were measured after transformation and antibiotic selection and analyzed with a camera-based method. Data points are the average of three separate experiments, and the error bars are indicative of the standard error