Fig. 4
Growth densities of Synechococcus sp. PCC 7002 wildtype and A0159, A0935 and A2842 strains. Growth density was calculated based on data obtained either by spectrophotometric measurements or by measuring spot intensities and related to the Synechococcus sp. PCC 7002 wildtype growth density. Statistical tests were performed with the unpaired t-test provided by Prism version 6 (GraphPad). Shown are the average of technical triplicates and error bars indicate the standard error. a Maximal growth density of A0159, A0935 and A2842 strains as a percentage of wildtype density in liquid medium. Mutated strains and wildtype Synechococcus sp. PCC 7002 were grown in liquid medium and OD730 measurements were performed every 24 h. Two-tailed P-values were 0.0018 (A2842), 0.0060 (A0935) and 0.0034 (A0159). b Maximal growth density A0159, A0935 and A2842 strains as a percentage of wildtype density on solid medium. Cultures of Synechococcus strains A0159, A0935 and A2842 were spot-plated and the relative change in intensity of the spots was assessed by a camera-based method. Wildtype Synechococcus sp. PCC 7002 was used as a control