Fig. 1
Monitored scattered light (a) and FbFP fluorescence intensity signals (b) during E. coli Tuner(DE3)/pRhotHi-2-LacI-EcFbFP cultures with varying times of induction (0.5–16 h) and concentrations of IPTG (0–1000 μM). a The time span of induction is highlighted by the gray area. For clarity, only 61 cultures of the entire data set (303 cultures, Additional file 1: Figure S1) are shown. b The final FbFP fluorescence intensity defines the color-coding. The weakest expression (≙ minimum final FbFP fluorescence intensity) is presented in blue and becomes more reddish with increasing final fluorescence intensity. The maximum final fluorescence intensity is presented in pure red. The 303 cultures were conducted in a total of eight MTPs. The investigated cultures are referred to as dataset A in Table 1. Cultivation conditions: 48 round deep-well MTP without optodes, VL = 800 μL, n = 1000 rpm, shaking diameter d0 = 3 mm, 30 °C