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Fig. 2 | Journal of Biological Engineering

Fig. 2

From: Prediction of Escherichia coli expression performance in microtiter plates by analyzing only the temporal development of scattered light during culture

Fig. 2

Analysis of scattered light intensity patterns applying principle component analysis (PCA). a For data pre-processing, the standard normal variation (SNV) at each scattered light intensity curve was calculated according to Eq. 5. The SNV transformed data of the 303 E. coli Tuner(DE3)/pRhotHi-2-LacI-EcFbFP cultures introduced in Fig. 1 was used for PCA. b Variance explained by each principle component (PC) of the entire dataset. c Scores of the second PC over the scores of the first PC. The color represents the final FbFP fluorescence intensity and corresponds to the color-coding in Fig. 1. The symbols represent the classification of the scattered light intensity pattern: no induction, insufficient induction (pattern close to non-induced culture), optimal induction (more or less linear course in Fig. 2a) and too strong induction (significantly lengthened lag-phase). The classification was performed manually. The investigated cultures are referred to as dataset A in Table 1

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