Fig. 4
Investigation of correlations between the final FbFP fluorescence intensity and parameters of the extended sigmoidal function (Eq. 1). In each plot, every symbol represents one complete E. coli Tuner(DE3)/pRhotHi-2-LacI-EcFbFP culture (dataset A). Four single cultures of the initial dataset of 303 cultures introduced in Fig. 1 were removed due to bad fit to the extended sigmoidal function. For all remaining 299 fits, regression coefficients (R2) higher than 0.96 are reached. The color represents the final FbFP fluorescence intensity and corresponds to the color-coding in Fig. 1. a-e Values of the five parameters (a-e) of the extended sigmoidal function for each culture (Eq. 1). f Calculated slope at time of the inflection point (parameter c) for each culture (Eq. 3). The slope is standardized according to Eq. 7. The black dotted line represents a linear regression (R2 = 0.66). Cultivation conditions: 48 round deep-well MTP without optodes, VL = 800 μL, n = 1000 rpm, shaking diameter d0 = 3 mm, 30 °C