Fig. 5

Classification of expression performance based on parameters obtained from fitting the extended sigmoidal function (Eq. 1) to scattered light intensity datasets. Standardized slope at the inflection point (\( {SL}_{fit}^{\prime }{(c)}_{Standardized} \); Eq. 7) is plotted over the standardized time at the inflection point (c _Standardized ; Eq. 6). Cultures were conducted under quite different conditions in two BioLector devices, two types of microtiter plates (48-well and 96-well) and at four temperatures (28 °C, 30 °C, 34 °C and 37 °C) with three different E. coli host strains (Tuner(DE3), BL21(DE3) and BL21-Gold(DE3)). The detailed culture conditions are given in Table 1. In total 621 different cultures are presented and each symbol represents one complete culture. Within each dataset, red represents the best expression performance while blue indicates bad expression performance. The cultures were classified in accordance to Fig. 2c in four clusters: no induction, insufficient induction, optimal induction and too strong induction. The specific ranges are indicated by ellipses