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Fig. 7 | Journal of Biological Engineering

Fig. 7

From: Investigation of poly(γ-glutamic acid) production via online determination of viscosity and oxygen transfer rate in shake flasks

Fig. 7

Measured (offline) and calculated (online) viscosity of all cultivations presented in this work. The offline effective viscosity was obtained via rheometer measurements of sampled cultivation broth. The online viscosity was calculated from the volumetric power input measured online via the power input shaker. Initial values: pH = 7.4, 80 g L−1 glycerol, 20 g L−1 glutamic acid, 12 g L−1 citric acid, trace elements and/or kanamycin. Cultivation conditions: T = 37 °C, 500 mL shake flasks, filling volume 100 mL, shaking frequency 250 rpm, shaking diameter 50 mm

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