Fig. 2From: Nucleotides upstream of the Kozak sequence strongly influence gene expression in the yeast S. cerevisiae Multiple point mutations to guanine. The ratio between the fluorescence level of the synthetic 5′-UTRs from k 26 to k 38 and that of k 1 are reported. The number of adenines or guanines in the upstream region is given below the leader sequence name (from k 27 to k 38). The subscripts −1, −2, and −3 indicate that an adenine is present in the extended Kozak sequence only at the corresponding position. Subscript i represents intermixed (see main text). Asterisks, p-value <0.05 vs. k 1 Back to article page