Fig. 5
Low MFE values are associated with reduced fluorescence expression. Red bars, difference between MFEs of the corresponding 5′-UTR and k 1 (ΔMFE). Blue bars, 10-fold magnified ratio between the fluorescence level of the indicated 5′-UTR and that of k 1. Apart from k 1, sequences are sorted by increasing ΔMFE. All sequences except k 4 contain multiple point mutations with respect to k 1. Asterisks above blue bars, p-value <0.05 vs. k 1