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Fig. 1 | Journal of Biological Engineering

Fig. 1

From: Three-dimensional (3D) evaluation of liquid distribution in shake flask using an optical fluorescence technique

Fig. 1

Liquid distribution measurement in shake flasks using an optical fluorescence technique. a shows a schematic diagram of the liquid distribution measurement setup for 250-mL shake flask. b shows a top view of the optical fiber arrangement at the side of the shake flask wall. c, d and e show a side view, close-up view and 45o distant view of the optical fiber arrangement to the shake flask, respectively. 1) Orbital shaker. 2) Hall sensor. 3) Magnet for triggering. 4) Light-emitting diode (LED) supplying excitation blue light (465 nm). 5) Optical fiber channeling the excitation blue light to the shake flask. 6) 45o angle distance of the optical fiber arrangement (seen from top view). 7) 250-mL shake flask clamped onto the orbital shaker’s table. 8) Enlarged view of the 14 different measurement positions to the side of the shake flask wall. Fourteen pairs of optical fibers were arranged vertically at these specified different heights from the base of the shake flask. 9) Optical fiber channeling the emission light (515 nm) from the shake flask to the photomultiplier tube (PMT). 10) Enlarged view of the bundle of 14 optical fibers. 11) Enlarged view of the PMT’s light sensitive area attached with a bundle of 14 optical fibers. 12) Data acquisition module (analog-to-digital converter). 13) Computer. 14) Brass holder for optical fibers. 15) Transparent core of optical fiber. 16) Shake flask wall. 17) Rotating fluorescent aqueous solution

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