Fig. 3
From: Construction of an easy-to-use CRISPR-Cas9 system by patching a newly designed EXIT circuit
Validation of the EXIT circuit with different plasmid replication origins and simultaneous plasmid elimination. a Robustness of the EXIT circuit with four different plasmid replication origins. N: not induced. b Scheme used for one-step elimination of two plasmids. c Efficiency determination for eliminating both pEC101 and pEM106 (bar represents standard error; n = 3 independent cultures). d Visual demonstration of pEC101 and pEM106 elimination on plates