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Fig. 11 | Journal of Biological Engineering

Fig. 11

From: Designing of PLA scaffolds for bone tissue replacement fabricated by ordinary commercial 3D printer

Fig. 11

Confocal microscopy photomicrographs of ST1 and ST2 seeded with osteosarcoma cells. Confocal microscopy photomicrographs of ST1 (a, c) and ST2 (b, d) scaffolds from polylactic acid seeded with osteosarcoma cells MG-63 after a 7-day and 14-day culture. Immunohistochemical staining using monoclonal antibody against either type I collagen (a, b) or osteocalcin (c, d), followed by secondary antibody conjugated with Alexa Fluor 488® (green) and propidium iodide staining of cell nuclei (red) showed groups of cells producing type I collagen on both scaffolds (a, b) after 7 days, but only rare osteocalcin staining in both scaffolds (c, d) after 14 days. Objective ×10×, magnification ×4, bar = 20 μm

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