Table 2 Platforms for configuring cells to replicate the BBB
Platforms for BBB modeling | Advantages | Disadvantages | References |
|---|---|---|---|
transwell model | • replicates confluent monolayer • suitable for basic co-culture • easy measurement of TEER and permeability • does not require pumps or microfabrication | • lacks shear stress • lacks cylindrical geometry • lacks proper heterotypic cell-cell contacts | |
membrane-based microfluidic models | • replicates shear stress • 2D plane allows for convenient imaging | • lacks cylindrical geometry and ECM | |
matrix-containing microfluidic models | • replicates shear stress • replicates a 3D environment for embedded cells • allows for some dynamic reorganization | • matrix can pose technical challenges, including contraction • lacks cylindrical geometry | [115] |
templated perfusable models | • replicates shear stress • replicates a 3D environment for embedded cells replicates cylindrical geometry | • difficult to fabricate < 20–50 μm diameter vessels • cannot measure TEER |