Fig. 1

Oxygen-limited cultures of E. coli expressing FbFP under control of P _kat . Online monitoring of DOT (a), pH (b), cell growth by scattered light (c) and FbFP fluorescence data are shown (d). Culture conditions: 48-well round well plate, n = 700 rpm, d₀ = 3 mm, V_L = 1500 (for OTR_max ca. 11 mmol L⁻¹ h⁻¹) or 2400 μL (for OTR_max ca. 7 mmol L⁻¹ h⁻¹), mineral medium buffered with MOPS (0.2 M) plus 5 g/L of glucose. Vertical lines show the standard deviation (n = 3) of average values