Fig. 3

Oxygen-limited cultures of E. coli expressing FbFP under control of the globin promoters. Online monitoring of DOT (a, b), pH (c, d), cell growth by scattered light (e, f) and FbFP fluorescence data are shown (g, h). Culture conditions: 48-well round well plate, n = 700 rpm, d₀ = 3 mm, V_L = 1500 (for OTR_max ca. 11 mmol L⁻¹ h⁻¹) or 2400 μL (for OTR_max ca. 7 mmol L⁻¹ h⁻¹), mineral medium buffered with MOPS (0.2 M) plus 5 g/L of glucose. Data from single experiments are shown for clarity