Fig. 3

Efficient removal of floxed-reporter genes with lentiviral transduction protocol. Functional validation of Cre-expression vectors using viral transduction. HEK293-TE cells at 20~40% confluency were transduced (0.5 MOI)with either lenti-Cre-GFP (a-c) or lenti-Cre-GFP-Puro (d-e) for 5 days and images were taken at 20× magnification. Green fluorescence indicates the expression of Cre-GFP or Cre-GFP-Puro (a, d). Red fluorescence indicates the expression of floxed-RFP-puro (b, e). Overlay of either A and B, or D and E, respectively, shows that the Cre-GFP expressing cells do not overlap with the floxed-RFP (c) or floxed-RFP-puro (f) cells. White arrows show GFP and RFP positivity are mutually exclusive. Scale bar 50 μm