Fig. 5
Efficiency of Salmonella vaccine delivery using the device. a Salmonella Typhimurium stationary phase cultures were exposed to shockwaves at different pressures to check the viability of bacteria; unexposed cultures served as the control group (b) Mice (n = 5 per group) were infected with lethal dose (108 CFU/mouse) of virulent Salmonella orally, 5 days after immunization as described in the previous experiment, and the survival of mice was estimated. [P < 0.0001 (Log rank test)]. c Single dose of DV-STM-07 was delivered using device, orally and through I.P route and the serum IgG levels were tested against Salmonella-specific Lipopolysaccharide (LPS) using ELISA; PBS delivered using the device was used as control; bar shows the mean value of the experiments. Error bar shows standard deviation (s.d.). (P value - Student’s t-test). d DV-STM-07 was administered to mice using the device and via oral route. Phosphate buffered saline (PBS) delivered using the device was used as control. Mice were infected with a lethal dose (107 CFU/mouse) of virulent strain of Salmonella at 1-week post-immunization. 5 days after the oral challenge, MLN, spleen and liver were aseptically dissected for checking the Salmonella burden. Statistical significance is specified as **P < 0.005 (Two-way ANOVA test). e–g Splenic T cells were isolated from unvaccinated and vaccinated mice. Cells were stained with PE-tagged antiCD8 antibody (Miltenyi Biotec) and were analyzed by flow cytometry. Numbers of CD8+ cells were compared between the unvaccinated and the vaccinated cohort