Fig. 3
From: Evaluation of green tea extract as a safe personal hygiene against viral infections
Maintenance of viral inactivation activity of GTE solution with supplements. 0.01, 0.05, and 0.1% GTE solutions were incubated at 25 °C (a) or 37 °C (b) in the presence of 2% citric acid, 0.1% sodium benzoate, and 0.2% ascorbic acid (GTE-mix). The GTE-mix solutions incubated for different times were mixed with 106 PF of PR8 virus for six h at 25 °C for viral inactivation. Residual viral titers were determined by plaque assay on MDCK cells. As a control, the virus was incubated with PBS, and the viral titer was indicated by upper dashed lines. c As another controls, the virus was treated with the individuals of the supplement or with the mixture of three agents. Data are the mean of two independent experiments. Detection limit of plaque assay is 5, and error bars indicate the SD