Fig. 4

Effects of supplements on the kinetics of viral inactivation by GTE. a Chemical stability of green tea catechins. 0.1% GTE and GTE-mix solutions were stored at 25 °C for up to 56 days. The samples were obtained at various time-points and catechins concentration was measure by GC. b Kinetics of viral inactivation by GTE and GTE-mix. 0.1% GTE and GTE-mix solutions were incubated at for 24 h at 25 °C. After the incubation, the GTE and GTE-mix solutions were mixed with 10⁶ PFU of PR8 virus and further incubated at 25 °C for 0, 1, 5, 10, 30, 120, and 360 mins for viral inactivation. Residual viral titers at each time-point were determined by plaque assay on MDCK cells. Data are the mean of two independent experiments. Detection limit of plaque assay is 5, and error bars indicate the SD