Skip to main content
Fig. 3 | Journal of Biological Engineering

Fig. 3

From: Synthetic pathways and processes for effective production of 5-hydroxytryptophan and serotonin from glucose in Escherichia coli

Fig. 3

Protein engineering of CtAAAH-F. a CtAAAH-F binding pocket predicted: the atoms of the cofactor and 2-His-1-caboxylate facial triad are present in gray, iron atom in pink; tryptophan surface is shown in blue, F197 and E219 atoms are present in orange and their surfaces are shown in green. b CtAAAH-LC binding pocket predicted: as in a., but L197 and C219 are present in orange, as well as their surfaces. c Lineweaver-Burk plot of CtAAAH-F and CtAAAH-LC. d tryptophan and 5HTP production after batch fermentation. Values in the graph are the average of triplicates, error bars correspond to the standard error of the mean (SEM)

Back to article page

Journal of Biological Engineering

ISSN: 1754-1611