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Fig. 3 | Journal of Biological Engineering

Fig. 3

From: Synthetic pathways and processes for effective production of 5-hydroxytryptophan and serotonin from glucose in Escherichia coli

Fig. 3

Protein engineering of CtAAAH-F. a CtAAAH-F binding pocket predicted: the atoms of the cofactor and 2-His-1-caboxylate facial triad are present in gray, iron atom in pink; tryptophan surface is shown in blue, F197 and E219 atoms are present in orange and their surfaces are shown in green. b CtAAAH-LC binding pocket predicted: as in a., but L197 and C219 are present in orange, as well as their surfaces. c Lineweaver-Burk plot of CtAAAH-F and CtAAAH-LC. d tryptophan and 5HTP production after batch fermentation. Values in the graph are the average of triplicates, error bars correspond to the standard error of the mean (SEM)

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