Table 2 List of strains and plasmids used for the production of 5-hydroxytryptophan and serotonin
Characteristics | Source | |
|---|---|---|
Strains | ||
S028 | W3110 ∆lacU169 gal490λCI857 ∆(cro-bioA) rpsL (StrR) ∆aroF∆aroG ∆mtr ∆tnaA ∆tnaB ∆aroH::PJ23119-rpsL-tac-(aroGS180F-serAH344A/N364A) Ptrc-trpES40FD CBA | Chen and Zeng, [14] |
TrpD-Pl | S028 ∆TrpR carrying pBbE1k-2.2 | |
TrpD-Gi | S028 ∆TrpR::PDC DHPR | |
10-β | New England Biolabs | |
BL21(DE3) | New England Biolabs | |
BL21(DE3) ∆tnaA | This study | |
Plasmidsa | ||
pACYCDuet-1 | Novagen | |
pCtAAAH-W192F | pACYCDuet-1; aromatic amino acid hydroxylase from Cupriavidus taiwanensis – W192F | Mora and Zeng [20] |
pACPJ23 | pACYCDuet-1.2, PT7 swapped with PJ23110 | This study |
pACPtrc | pACYCDuet-1.2, PT7 swapped with Ptrc | This study |
pACPJ23-CtAAAH-F | pACPJ23; aromatic amino acid hydroxylase-W192F from Cupriavidus taiwanensis | This study |
pACPtrc-CtAAAH-F | pACPtrc; aromatic amino acid hydroxylase-W192F from Cupriavidus taiwanensis | This study |
pBbE1k-2 | pBbE1k; pterin-4 alpha-carbinolamine dehydratase (PCD) and dihydropteridine reductase (DHPR) from human | Satoh et al., [42] |
pSentrp | Intracellular L-tryptophan sensor | Fang et al., [31] |
PSentrp(-LVA) | pSentrp; GFP with protease signal | This study |
pCOLADuet- | pCOLADuet-1; GSTΔ37 tryptamine | Park et al.,[26] |
GSTΔ37T5H + TDC | hydroxylase and tryptophan decarboxylase | |
pCOLADuet-TDC | pCOLADuet-1; tryptophan decarboxylase | This study |
pBAD/His | ThermoFisher Scientific | |
pBAD-TDC | pBAD/His; tryptophan decarboxylase | This study |
pKD46 | Red recombinase expression plasmid from phage λ, temperature sensitive plasmid | Datsenko and Wanner, [34] |
pCP20 | FLP recombinase, temperature sensitive plasmid | Datsenko and Wanner, [34] |