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Table 2 List of strains and plasmids used for the production of 5-hydroxytryptophan and serotonin

From: Synthetic pathways and processes for effective production of 5-hydroxytryptophan and serotonin from glucose in Escherichia coli

  Characteristics Source
Strains
 S028 W3110 ∆lacU169 gal490λCI857 ∆(cro-bioA) rpsL (StrR) ∆aroF∆aroG ∆mtr ∆tnaA ∆tnaB ∆aroH::PJ23119-rpsL-tac-(aroGS180F-serAH344A/N364A) Ptrc-trpES40FD CBA Chen and Zeng, [14]
 TrpD-Pl S028 ∆TrpR carrying pBbE1k-2.2  
 TrpD-Gi S028 ∆TrpR::PDC DHPR  
 10-β   New England Biolabs
 BL21(DE3)   New England Biolabs
 BL21(DE3) ∆tnaA   This study
Plasmidsa
 pACYCDuet-1   Novagen
 pCtAAAH-W192F pACYCDuet-1; aromatic amino acid hydroxylase from Cupriavidus taiwanensis – W192F Mora and Zeng [20]
 pACPJ23 pACYCDuet-1.2, PT7 swapped with PJ23110 This study
 pACPtrc pACYCDuet-1.2, PT7 swapped with Ptrc This study
 pACPJ23-CtAAAH-F pACPJ23; aromatic amino acid hydroxylase-W192F from Cupriavidus taiwanensis This study
 pACPtrc-CtAAAH-F pACPtrc; aromatic amino acid hydroxylase-W192F from Cupriavidus taiwanensis This study
 pBbE1k-2 pBbE1k; pterin-4 alpha-carbinolamine dehydratase (PCD) and dihydropteridine reductase (DHPR) from human Satoh et al., [42]
 pSentrp Intracellular L-tryptophan sensor Fang et al., [31]
 PSentrp(-LVA) pSentrp; GFP with protease signal This study
 pCOLADuet- pCOLADuet-1; GSTΔ37 tryptamine Park et al.,[26]
 GSTΔ37T5H + TDC hydroxylase and tryptophan decarboxylase  
 pCOLADuet-TDC pCOLADuet-1; tryptophan decarboxylase This study
 pBAD/His   ThermoFisher Scientific
 pBAD-TDC pBAD/His; tryptophan decarboxylase This study
 pKD46 Red recombinase expression plasmid from phage λ, temperature sensitive plasmid Datsenko and Wanner, [34]
 pCP20 FLP recombinase, temperature sensitive plasmid Datsenko and Wanner, [34]