Fig. 2
Calibration of CNT@DNA-Mn3(PO4)2 modified glass-carbon electrode for measurement of O2•− and electrochemical characterization of O2•− released by cells following UVR irradiation. a Cyclic voltammetry (CV) curves measured with PBS and PBS plus different concentrations of KO2 (nM). a: 0, b: 5, c: 10, d: 20, e: 40, f: 60, g: 80, h: 100, i: 150, j: 200; inset: CV curves measured with PBS (black), PBS plus KO2 (150 nM, blue), and KO2 solution added with SOD (300 U mL− 1, red). b Calibration curve for serial concentrations of O2•−. c O2•− CV curves of cells under irradiation, a. UV, b. UVA, c. UVB, d. Sham-irradiation control. d Histogram of peak current changes compared to sham-irradiated cells (black column, n = 3) and generation of O2•− upon irradiation with UV as quantified by the O2•−assay kit: increase of absorbance at 450 nm (grey column, n = 3). SOD: superoxide dismutase. UV (10.5 J cm− 2 = 105 kJ m− 2), UVA (10 J cm− 2 = 100 kJ m− 2), UVB (0.5 J cm− 2 = 5 kJ m− 2)