|MTT assay||Fluorescent assay||Electrochemical detection|
|Assay time||24–72 h||1-2 h||Within minutes|
Alive cell catalyse enzyme to indirectly characterize cell growth.|
Evaluate the overall effect of treatment on cell survival, growth.
Fluorophore probe is used to label target molecules.|
Not feasibly for real-time characterizing a reaction.
Staining process will affect the health of cells.
In site characterize a reaction.|
No damage to cells.
Fast reaction time allows for high throughput screening.
No expensive equipment is involved.