Fig. 1

Comparison of CP color development. a LB chloramphenicol agar plates incubated at 37 °C for 20 h comparing (left, clockwise starting from 12 o’clock) meffBlue, aeBlue, cjBlue, amilGFP, fwYellow, amajLime, scOrange, amilCP and (right, clockwise starting from top) gfasPurple, eforRed, asPink (asCP), meffRed, tsPurple and spisPink expressed from a high-copy plasmid. b Comparison of cjBlue and meffRed with similarly-colored CPs after full color development = 37 °C for four days (clockwise starting from 12 o’clock: control promoter-less aeBlue that also lacked a ribosome binding site, scOrange, meffRed, eforRed, spisPink, meffBlue, cjBlue and aeBlue). c Time dependence of the color of aeBlue. A single plate is shown after: 19 h at 37 °C, then additional 4 °C incubations for one, two and three days (left to right, respectively)