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Fig. 2 | Journal of Biological Engineering

Fig. 2

From: Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology

Fig. 2

Variation in color intensity with CP expression. a Effect of plasmid copy number (using J23110 promoter). Medium copy plasmid indicates pSB3K3; high copy, pSB1K3. Plates were incubated for 20 h. b Effect of promoter strength and temperature (using high copy plasmid). Left side of plates: medium/low promoter BBa_J23116 (396 arbitrary units [16]). Right side of plates: medium promoter BBa_J23110 (844 arbitrary units [16]). Plates were incubated for 23 h at the temperatures indicated followed by 22 h at room temperature. The median intensity above background of colonies +/− the standard deviation is given above each plate half (see Additional file 1: Figure S1)

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