Table 1 Putative functions of the genes in the chromomycin biosynthetic gene cluster
Gene | Annotation | Size (aa) | Homologue | Identities |
|---|---|---|---|---|
srcmY | cyclase | 258 | DacN [Dactylosporangium sp. SC14051] | 70% |
srcmTI | ketoreductase | 258 | CmmTI [Streptomyces griseus subsp. griseus] | 64% |
srcmS | acyl carrier protein | 85 | Acyl carrier protein [Streptomyces sp. 275] | 100% |
srcmV | NDP-hexose 2,3-dehydratase | 462 | PnxS3 [Streptomyces sp. TA-0256] | 54% |
srcmW | NDP-hexose 3-ketoreductase | 331 | PokS4 [Streptomyces diastatochromogenes] | 53% |
srcmUII | NDP-hexose 4-ketoreductase | 252 | SaqR [Micromonospora sp. Tu 6368] | 53% |
srcmOI | FAD-dependent monooxyngease | 423 | CmmOI [Streptomyces griseus subsp. griseus] | 70% |
srcmX | cyclase | 142 | DacG [Dactylosporangium sp. SC14051] | 60% |
srcmK | chain length factor | 402 | SaqB [Micromonospora sp. Tu 6368] | 66% |
srcmP | ketosynthase | 422 | SaqA [Micromonospora sp. Tu 6368] | 76% |
srcmE | dTDP-glucose 4,6-dehydratase | 326 | CmmE [Streptomyces griseus subsp. griseus] | 75% |
srcmD | NDP-glucose synthase | 355 | Lct49 [Streptomyces rishiriensis] | 57% |
srcmMIII | sugar O-methyltransferase | 252 | AlmCII [Streptomyces sp. A1] | 61% |
srcmRI | SARP family transcriptional regulator | 292 | CmmRI [Streptomyces griseus subsp. griseus] | 71% |
srcmMII | C-methyltransferase | 343 | mtmMII [Streptomyces argillaceus] | 57% |
srcmUI | NDP-hexose 4-ketoreductase | 254 | ChaR [Streptomyces chattanoogensis] | 54% |
srcmMI | O-methyltransferase | 344 | CtcO [Kitasatospora aureofaciens] | 51% |
srcmGI | glycosyltransferase | 394 | PyrC4 [Streptomyces rugosporus] | 41% |
srcmOIV | FAD-dependent monooxygenase | 511 | mtmOIV [Streptomyces argillaceus] | 55% |
srcmWI | side-chain ketoreductase | 326 | MtmW [Streptomyces argillaceus] | 64% |
srcmLI | acyl CoA ligase | 409 | CmmLI [Streptomyces griseus subsp. griseus] | 68% |
srcmH | ABC transporter membrane protein | 251 | SfrB [Streptomyces sp. 275] | 100% |
srcmI | ABC transporter ATP-binding protein | 322 | SfrA [Streptomyces sp. 275] | 100% |
srcmRII | PadR family transcriptional regulator | 186 | CmmRII [Streptomyces griseus subsp. griseus] | 77% |
srcmJ | Excinuclease ABC subunit A | 825 | CmrX [Streptomyces griseus subsp. griseus] | 79% |
srcmC | NDP-hexose C-methyltransferase | 409 | CmmC [Streptomyces griseus subsp. griseus] | 78% |
srcmUIII | 4-ketoreductase | 335 | StaK [Streptomyces sp. TP-A0274] | 50% |
srcmGIII | glycosyltransferase | 400 | ChlC7 [Streptomyces antibioticus] | 41% |
srcmA | acetyltransferase | 422 | CmmA [Streptomyces griseus subsp. griseus] | 74% |
srcmGIV | glycosyltransferase | 391 | PyrC4 [Streptomyces rugosporus] | 41% |
srcmGII | glycosyltransferase | 391 | SsfS6 [Streptomyces sp. SF2575] | 38% |
srcmF | dTDP-4-keto-6-deoxy-D-glucose epimerase | 199 | dTDP-4-keto-6-deoxy-D-glucose epimerase [Streptomyces tsukubensis] | 78% |
srcmOII | oxygenase | 550 | DacO2 [Dactylosporangium sp. SC14051] | 57% |
srcmTII | ketoreductase | 253 | RubJ [Streptomyces collinus] | 50% |
srcmQ | aromatase | 320 | DacK [Dactylosporangium sp. SC14051] | 45% |
srcmLII | acyl CoA ligase | 536 | SsfL2 [Streptomyces sp. SF2575] | 48% |